Coding

Part:BBa_K588000:Design

Designed by: Kevin Lu   Group: iGEM11_Rutgers   (2011-09-26)

trpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


Design Notes

Cloned from pJPR2 by PCR. RFC10 biobrick prefix and suffix added.

Forward Primer 5'- atcaatgaattcgcggccgcttctagatggcccaacaatcaccc -3'

Reverse Primer 5'- taactactgcagcggccgctactagtatcaatcgcttttcagc -3'

Source

Plasmid pJPR2 provided by Janet Paluh, described in Paluh and Yanofsky

References

Paluh, Janet L. and Charles Yanofsky. High level production and rapid purification of the E. coli trp repressor. Nucleic Acids Research 14. 20 (1986):7851-7860